Inhibitory activities of aromatic amino acid esters and peptides against ovalbumin permeation through Caco-2 cell monolayers.
نویسندگان
چکیده
Trp, Phe, and Tyr ethyl esters and their dipeptides with Gly at the C-terminals inhibited ovalbumin (OVA) permeation through Caco-2 monolayers. The inhibitory activity of Trp ethyl ester was the highest at near the concentration of 10(-6) M. It was suggested that Trp ethyl ester inhibited transcellular permeation of OVA.
منابع مشابه
Effect of Aliphatic and Aromatic Thiolated Chitosan Conjugates on Permeability of Ritonavir across Caco-2 Cell Monolayers and Rat Intestinal Membrane
It was the aim of this study to determine the potential of two aliphatic and two aromatic thiolated chitosan conjugates: chitosan-thioglycolic acid (chitosan-TGA), chitosan-4-thiobutylamidine (chitosan-TBA), chitosan-6mercaptonicotinic acid (chitosan-6MNA) and chitosan-4-mercaptobenzoic acid (chitosan-4MBA), respectively, as permeation enhancing agents. Ritonavir was employed as model compound....
متن کاملIsolation of tryptophan as an inhibitor of ovalbumin permeation and analysis of its suppressive effect on oral sensitization.
Tryptophan was isolated from rat feces as an active compound against ovalbumin permeation in an in vitro Caco-2 cell model. Tryptophan dose-dependently inhibited ovalbumin permeation with accompanying increase in transepithelial electric resistance, and its inhibitory activity reached a plateau at 10 mM. Brown Norway rats were sensitized by intragastric administration of ovalbumin together with...
متن کاملVasoactive intestinal peptide reduces H-coupled amino acid uptake across the apical membrane of human intestinal Caco-2 cell monolayers
School of Cell & Molecular Biosciences, The Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH, UK H-coupled b-alanine uptake across the apical membrane of intestinal epithelial (Caco-2) cell monolayers (when measured at apical pH 6.5) is inhibited by forskolin but not 1,9dideoxyforskolin suggesting modulation by a cAMP-dependent pathway (Anderson et al. 2001). The purpose of this s...
متن کاملEnzyme-modified cheese exerts inhibitory effects on allergen permeation in rats suffering from indomethacin-induced intestinal inflammation.
We have found that an enzyme-modified cheese (EMC) inhibited the permeation of allergens such as ovalbumin (OVA), using Caco-2 cells as an in vitro intestinal epithelial model. In addition, NPWDQ (Asn-Pro-Trp-Asp-Gln, aa 107-111 of alphas(2)-casein) was isolated from EMC and identified as one of the responsible peptides for this inhibitory activity (Tanabe et al., J. Agric. Food Chem., (2007))....
متن کاملArctigenin from Fructus Arctii (Seed of Burdock) Reinforces Intestinal Barrier Function in Caco-2 Cell Monolayers
Fructus Arctii is used as a traditional herbal medicine to treat inflammatory diseases in oriental countries. This study aimed to investigate effect of F. Arctii extract on intestinal barrier function in human intestinal epithelial Caco-2 cells and to reveal the active component of F. Arctii. We measured transepithelial electrical resistance (TEER) value (as an index of barrier function) and ov...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 67 11 شماره
صفحات -
تاریخ انتشار 2003